Evidence confirms an anthropic origin of Amazonian Dark Earths

Umberto Lombardo 1,2✉, Manuel Arroyo-Kalin 3✉, Morgan Schmidt 4, Hans Huisman5, Helena P. Lima 6, Claide de Paula Moraes 7, Eduardo G. Neves8, Charles R. Clement9, João Aires da Fonseca10, Fernando Ozorio de Almeida 11, Carlos Francisco Brazão Vieira Alho 12, Christopher Bronk Ramsey 13, George G. Brown 14, Marta S. Cavallini8, Marcondes Lima da Costa 15, Luís Cunha 16, Lúcia Helena C. dos Anjos17, William M. Denevan18, Carlos Fausto19,20,

F irst described over 120 years ago in Brazil, Amazonian Dark Earths (ADEs) are expanses of dark soil that are exceptionally fertile and contain large quantities of archaeological artefacts. The elevated fertility of the dark and often deep A horizon of ADEs is widely regarded as an outcome of pre-Columbian human influence 1 . Archaeological research provides clear evidence that their widespread formation in lowland South America was concentrated in the Late Holocene, an outcome of sharp human population growth that peaked towards 1000 BP 2-4 . In their recent paper Silva et al. 5 argue that the higher fertility of ADEs is principally a result of fluvial deposition and, as a corollary, that pre-Columbian peoples just made use of these locales, contributing little to their enhanced nutrient status.
Soil formation is inherently complex and often difficult to interpret, requiring a combination of geochemical data, stratigraphy, and dating. Although Silva et al. use this combination of methods to make their case 5 , their hypothesis, based on the analysis of a single ADE site and its immediate surroundings (Caldeirão, see maps in Silva et al. 5 ), is too limited to distinguish among the multiple possible mechanisms for ADE formation. Moreover, it disregards or misreads a wealth of evidence produced by archaeologists, soil scientists, geographers and anthropologists, showing that ADEs are anthropic soils formed on land surfaces enriched by inputs associated with pre-Columbian sedentary settlement [6][7][8][9] . To be accepted, and be pertinent at a regional level, Silva et al.'s hypothesis 5 would need to be supported by solid evidence (from numerous ADE sites), which we demonstrate is lacking. the parent material of the ADE and adjacent Ultisol shows analogous clay mineralogy and geogenic composition: both sites are characterised by the same 1:1 clays (as shown by Silva et al.'s Supplementary Fig. 3 5 ) and both lack the 2:1 clay minerals expected from fluvial origin 12 . Moreover, no difference is observed in the geogenic elements (Al, Ti, Cr, V, Fe, As) (Fig. 1A). Third, the overall mineral assemblage of the Caldeirão ADE is incompatible with the geochemistry of the sedimentary load of the Solimões River (Fig. 1A, B, D). Fourth, the lower clay content in the anthropic ADE horizons at Caldeirão (erroneously described by Silva et al. as "sandy clay loam" 5 ) is not evidence of fluvial deposition but a partial outcome of argilluviation 9 . Fifth, other well-studied ADE sites nearby contradict Silva et al.'s inference 5 : at the Hatahara ADE site, located 4 km from Caldeirão on the same Miocene bluff, the similarity in quartz sand grain morphology between the ADE A and B horizons excludes the inference of fluvial inputs into the A horizon 13 . Further afield, a large number of ADE sites are found along blackwater (non alluvial) rivers, associated with small headwater streams and springs, or found at elevations exceeding 90 m above the maximum flood level [14][15][16] , demonstrating that alluvial deposition is irrelevant to the formation of many ADE expanses. Indeed, if ADE were the result of alluvial processes, their spatial distribution along rivers would be continuous rather than patchy.

Archaeological considerations
Research conducted at numerous archaeological sites in the Central Amazon 17 has shown that the largest ADE expanses record multicomponent occupations that date to the period 1200-800 BP and are often underlain by remains of older (<2500 BP) ceramic occupations [2][3][4]6 . This also applies in the case of the Caldeirão site, where coring and excavations clearly show that the ADE is a pottery-rich archaeological deposit characterised by a predominantly human-made assemblage of mounds and pits ( Fig. 2A-E). Silva et al.'s sampling transects and elemental/isotopic measurements neither take into consideration nor detect this demonstrable anthropic conditioning of pre-Columbian origin (see Inset II in Fig. 2E) 5 . Furthermore, Silva et al. misunderstand stratigraphic associations when suggesting that >7.6 ky 14 C BP charcoal collected from −90 cm in their Ultisol transect provides an accurate age marker for the beginning of ADE formation 5 . Middle Holocene charcoal fragments are commonly found stratified in Amazonian soil profiles 18 , including the B horizons of ADE profiles 14 . However, the relevant age to understand ADE formation (and whether it is consistent with human occupation) is that of the silt-sized charcoal making up the dark horizon of an ADE. At the nearby ADE site of Hatahara, the age of this charcoal pool is consistent with a late first millennium AD Paredão phase settlement, albeit with older occupations starting around 500 BC 19,20 . For Caldeirão, similar ages have been reported 21 .

Demographic considerations
Silva et al. argue that a late Holocene onset for incipient agriculture in the Central Amazon region would preclude populations large enough to produce the levels of elemental enrichment recorded at Caldeirão 5 . This argument presupposes that indigenous land use regimes relying on incipient agriculture, aquatic wildlife, and hunting could not have created areas of persistent high fertility. This assumption does not account for decades of research on the subject. For instance, ethnoarchaeological research with the Kuikuro community, who are fisher-cultivators that live in the Upper Xingu region, has demonstrated that the greatest enrichment in P, Ca, and Sr, as well as high organic carbon and nearly neutral pH, occurs in mounded refuse middens. Once enriched soil horizons form in the middens, typically within a few years, they are often used for cultivating crops such as maize, sweet potato, and manioc 22 . Soil enrichment and ADE formation, therefore, are consistently associated with domestic activities in indigenous villages and, contrary to Silva et al.'s claim 5 , it is this elemental enrichment accumulating in settlements that is used for cultivation (and not the other way around). More broadly, measurements of elemental enrichment with P and Ca constitute a poor demographic proxy and, on their own, do not reveal agricultural activity: virtually any long human occupation can result in soil enrichment 23 . ADE sites, like Caldeirão, are very rich in nutrients because they concentrate human debris and waste associated with resources gathered or produced in large areas. It is the concentration of resources in settlements that produce ADEs over hundreds or thousands of years. Put another way, a thousand people could extract resources produced from a 50 hectares' catchment but concentrate debris and waste in a village of 0.1 hectares. Silva et al.'s 5 reference to improbably large agricultural populations, which implicitly suggests that ADEs were initially established for agricultural purposes, does not constitute evidence of fluvial deposition and disregards the association between ADE and middens that is supported by current research.
Elemental enrichment and isotopic ratios of ADE vs. Ultisols (Acrisols) Most of the co-authors of Silva et al. 5 have elsewhere argued that the elemental composition of Caldeirão site "…can be used to unveil ADE sites and differentiate them from Amazonian soils without anthropic influence" 24 . We agree with their earlier assessment: enrichment of the ADE compared to the Ultisols is consistent with inputs associated with human settlement. Among the latter are those related to burning, including K, Rb, Ba, Ca, Sr, P (from ash and charcoal); P, Ca, Sr, K, Zn, Cu (human waste); and Ca, P, Sr, Zn (bone debris) (Fig. 1B, C) 25 . Most of these, along with pyrogenic C, have been reported in ADEs 8 . The most logical explanation for such an assemblage is anthropic inputs associated with settlement activity. Indeed, research at the Hatahara site shows that the dark ADE sediments are bulked up by sand and silt-sized particulate material resulting from anthropic activity (fragmented charcoal and bone, pottery fragments, sponge spicules, etc.) 13 . Bioturbation can then mix these added materials in soil over time throughout the profile. How, then, can a fluvial input be surmised? The core of Silva et al.'s argument is that differences in Sr and Nd isotope ratios between ADE and Ultisols are best explained by fluvial inputs 5 . However, both Sr and Nd are found in plants 26 and terrestrial and aquatic vertebrates 27 , as well as in mineral matter and Silva et al. admit that their methods cannot discriminate these sources 5 . As there are no independent indications of sediment input in ADE's bulk chemical composition, but ample evidence for non-mineral anthropogenic inputs, it is most likely that isotopic signature in the studied ADE resulted from the deposition of food debris. Silva et al. regard the difference in elemental stoichiometries of freshwater fish (Ca:P 2.13) and human faeces (Ca:P~2) compared with ADEs as further evidence of ADE being of fluvial origin 5 . However, while the Ca:P ratio is highly variable in Caldeirão ADE (Fig. 1C), the modern Ca:P ratio in ADEs is the result of differential preservation coupled with the specific tropical soil dynamics of Ca, which is easily leached, and P, which binds with soil Fe and Al oxides 28 .
By way of conclusion: the geogenic model for ADE formation, which famously argued that ADEs are dark soils of natural fertility resulting from the deposition of alluvial horizons 10 , was laid to rest over 40 years ago 29 . Silva et al.'s hypothesis 5 reiterates this geogenic position but, as we have shown here, it does not stand up to scrutiny.

Data availability
All relevant data are provided with the paper.